A stool gram stain is a laboratory test that uses different stains to detect and identify bacteria in a stool sample.

The gram stain method is one of the most commonly used techniques for the rapid diagnosis of bacterial infections.

You will need to collect a stool sample.

There are many ways to collect a sample. You can catch the stool on plastic wrap that is loosely placed over the toilet bowl and held in place by the toilet seat, and then place the sample in a clean container. (One test kit supplies a special toilet tissue that you use to collect the sample.) Do not take stool samples from the toilet bowl water, because this can cause errors.

If you need to collect a sample from a child still in diapers, line the diaper with plastic wrap. Position the plastic wrap so that it keeps the stool from any urine. Mixing of urine and stool can spoil a good sample.

Your health care provider will give you instructions on when and how to return the sample.

The sample is sent to a laboratory. A small amount is spread in a very thin layer to a glass slide. This is called a smear. A series of special stains are added to the sample. The lab team member examines the stained smear under the microscope, looking for bacteria. The color, size, and shape of the cells help identify the specific bacteria.

A lab smear is painless and does not involve the patient.

There is no discomfort when a stool sample is collected at home because it only involves normal bowel functions.