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Protein electrophoresis - serum

Protein electrophoresis - serum

Blood test
Blood test

Definition:

This test roughly measures the types of protein in the fluid (serum) part of a blood sample.

See also:

How the test is performed:

The health care provider draws blood from a vein, usually on the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the band to swell with blood.

A needle is inserted into the vein, and the blood is collected in an airtight vial or a tube attached to the needle (syringe). During the procedure, the band is removed to let blood flow again. Once the blood has been collected, the needle is removed. The puncture site is covered to stop any bleeding.

For an infant or young child, the area is cleaned with antiseptic and punctured with a sharp needle or a small blade (lancet). The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.

Electrophoresis is a laboratory technique. The blood serum is placed on specially treated paper and exposed to an electric current. The various proteins move on the paper (migrate) to form bands that show the proportion of each protein fraction.

Individual proteins, except albumin, are not usually measured. However, protein fractions or groups ARE measured. The levels of protein fractions can be roughly measured by measuring the total serum protein and then multiplying that by the relative percentage of each protein fraction.

Lipoprotein electrophoresis is a type of protein electrophoresis that determines the amount of chemical compounds made up of protein and fat, called lipoproteins (such as LDL cholesterol).

How to prepare for the test:

The health care provider may ask you to stop taking drugs that could affect the test. DO NOT stop taking any medication without first asking your health care provider.

You may be asked to fast for 4 hours before a lipoprotein electrophoresis test.

How the test will feel:

When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging sensation. Afterward, there may be some throbbing.


Review Date: 3/8/2007
Reviewed By: Rita Nanda, M.D., Department of Medicine, Section of Hematology/Oncology, University of Chicago Medical Center, Chicago, IL. Review provided by VeriMed Healthcare Network.

The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed physician should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. Copyright 2002 A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.

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